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Biologist_PhD_15 years of experience

I am currently available for work
Serial No: 73108
(22/10/1982, male)
List top 5 skills: biologist
Qualifications and Skills:

Cell Culture
• Culture and storage of mammalian (cancer) adherent and suspension cell lines
• Culture and storage of insect cell lines
• Isolation, culture and storage of human & mouse primary BM MSC
• Isolation and culture of mouse primary ECN, mouse cortical astrocytes and adult mouse SVZ- NPC
• Isolation, culture and storage of mouse primary tail fibroblasts
• Transwell coculture models
• Scratch wound assay (in vitro cell migration assay)
• Assessment of neuron death and survival (LDH, WST-1, MTT assay)
Molecular Biology and Biochemistry
• DNA, RNA and protein isolation from cells, tissues and human biospecimens
• Reverse Transcription PCR & Real-Time PCR
• Western blot
• Southern blot
• cDNA microarrays
• Slot blot
• Enzyme-linked Immunosorbent Assay (ELISA)
• Plasmid DNA purification
• Restriction enzyme digestion of DNA
Animal Models
• Characterization of transgenic and knockout mouse models
• Mouse husbandry and genotyping
• Mouse behavior monitoring
• Mouse tissue dissection and banking
• Histology (cryostat)
• Immunohisto- and Immunocytochemistry
• Induction and clinical scoring of CNS disease mouse models (multiple sclerosis-EAE and status epilepticus-kainic acid induced)
Microbiology
• Detection and isolation of viral strains (enteroviruses, measles virus, rubella virus & cytomegalovirus) from human biospecimens by cell culture
• Detection and isolation of enteroviruses from sewage samples by cell culture
• Detection of pathogen-specific antibodies in human serum specimens by immunofluorescence, microneutralization assay (CCID50), Western blot and ELISA
• Culture and storage of laboratory Helicobacter pylori strains
• Culture, antimicrobial susceptibility testing and storage of clinical Helicobacter pylori strains
Bright field and fluorescence microscopy
IT
• Statistical software- Sigma Plot, SigmaStat, GraphPad Prism
• PC applications- MS office, Adobe Photoshop etc.

ADDITIONAL SKILLS

• Study design, data statistical analysis, results interpreting
• Experience in writing and submitting papers for publication, preparing grant proposals, giving talks and poster presentations
• Familiarity with record-keeping
• Experience in working in Biological Safety Levels -2 and -3
• Training and supervision of undergraduate, MSc and PhD students

LANGUAGE SKILLS

• Greek (native speaker)
• English (fluent)
• German (good command)
• Italian (basic communication skills)

Previous Employment Details:

Staff Scientist
June 2015-present
Diagnostic Service Department & National Polio/Enteroviruses Reference Laboratory (accredited by WHO), headed by Dr. A. Mentis, Hellenic Pasteur Institute, Athens, Greece

European Committee for Treatment and Research of Multiple Sclerosis (ECTRIMS) Postdoctoral Research Fellow
May 2013-May 2015
Laboratory of Neuroimmunobiology, headed by Prof. A. Uccelli
University of Genoa, Genoa, Italy

Staff Scientist
November 2009-April 2013
Diagnostic Service Department & National Polio/Enteroviruses Reference Laboratory (accredited by WHO), headed by Dr. A. Mentis
Hellenic Pasteur Institute, Athens, Greece

Ph.D. Student
April 2006-April 2012
Laboratory of Molecular Genetics, headed by Dr. L. Probert
Hellenic Pasteur Institute, Athens, Greece

Current location: 

Decentralized Administration of Attica, Greece - View on map

Nationality: 

Ελλάδα

Preferred Sector of Employment: 

Pharmaceutical and Science

Spoken languages: 

english, german, Greek, italian

Locations I am interested in working:

Europe